Volume 1 Issue 1
Recent Advances in Selection and Engineering of Biomass Degrading Enzymes
Alexander V. Gusakov *
Plant biomass represents a renewable and practically inexhaustible bio resource for production of fuels and chemicals, and in the coming years it is considered to play the same role in human civilization as oil in 20th and beginning of the 21st century. For the last three decades, the production of first-generation liquid biofuels (mainly, ethanol from food crops in the USA and that from sugarcane in Brazil) has continuously grown.
Molecular Dynamic Simulation and Free Energy of Binding Analysis of Novel Compounds from ZINC Database against Wild Type and Engineered Mutant (C1191I, C1191K)DNA Methyltransferase 1
Arunima Shilpi, Sabnam Parbin, Dipta Sengupta, Swayamsiddha Kar, Moonmoon Deb, Sandip Kumar Rath, Nibedita Pradhan and Samir Kumar Patra*
DNA methyltransferase 1 (DNMT1) has emerged as a potential epigenetic target for development of novel anticancer drugs. In the present investigation, we elaborate systematic virtual screening of large collection of natural products as DNMT1 inhibitors. The virtual screening was executed by implementing several docking programs, such as Glide_XP, Autodock, CDOCKER and LigandFit. Screening of 5120 commercially available FDA-approved drugs (mostly phytochemicals) from ZINC database ranked ZINC38517271, ZINC70455592 and ZINC31983781 as top 3 hits with respective Glide score as high as -14.33, -14.18 and -13.08 kcal/mol.
Improvement of Bacillus pumilus for Higher Xylanase Production by Mutation
Ranganathan Kapilan*, Vasanthy Arasaratnam
Mutation can cause a raise in the enzyme production by the favourable changes in the genotype of a bacterial strain. The objective of the study was to obtain a better xylanase producing Bacillus pumilus mutant strain by exposing the wild-type strain to UV-irradiation, heat shock and EMB chemical treatment. When Bacillus pumilus was subjected to UV-irradiation for 3 cycles, xylanase production was increased by 1.22 times in the first two cycles and there was no increase in the third cycle.
Purification of Extracellular xylanase from Bacillus subtilis BS166 by Affinity Precipitation Using Eudragit S-100
This study was aimed to compare the purification of xylanase produced by Bacillus subtilis BS166 with three phase partitioning and precipitation methods using Eudragit S 100 and to optimize these methods to increase the purification process. In the three phases partitioning method 13.9 UmL-1 of xylanase activity was obtained with a specific activity of 30.22 Umg-1. The recovery of the enzyme was 52.3 % with 1.74 fold purification. In the precipitation method, 15.6 UmL-1 of xylanase activity was obtained with the specific activity of 36.28 Umg-1.
Enzyme Production by Rhizophus Stolonifer Isolated from Bread and Kinetic Properties of the Extracellular Amylase
Rhizophus species are widely distributed thread like mold, categorized under Kingdom Fungi. It is capable of producing diverse group of enzymes including amylases, proteases and xylanases. The study was aimed to determine the kinetic properties and stability of amylase enzyme produced by Rhizophus stolonifer strain purified from bread. A Rhizophus stolonifer strain than can grow and produce amylase enzyme on moist bread at room temperature at neutral pH, was selected for this study and screened for the enzyme production.
Purification and Partial Characterization of a Phenol Oxidase from the Edible Mushroom Auricularia Fuscosuccinea
Yanez-Montalvo A, Vazquez-Duhalt R, Cruz-López L, Calixto-Romo MA, Sánchez JE*
A phenol oxidase from Auricularia fuscosuccinea was purified and partially characterized. Extracellular enzyme phenol oxidase was purified up to 55.9-fold from the culture filtrate by a protocol of three steps, ammonium sulfate precipitation twice (50 and 80% w/v), then two columns of ion exchange chromatography, first a DEAE-cellulose column and finally a highaffinity resin column.