Articles in Press
Volume 3 Issue 1
Molecular Cloning, Overexpression, Purification, Immobilization and Characterization of Lipase from Staphylococcus pasteuri
Magdy M Yousef, H Elsawy*
Thermostable lipase from Staphylococcus pasteuri was cloned, purified, immobilized and characterized. In this study, lipase gene was expressed in Escherichia coli and purified by DEAE-Sepharose and Glutathione S-sepharose 4B gel column chromatography. Lipase enzyme was purified to homogeneity by 28 times with a final yield of 175 %. The molecular weight of the purified enzyme was estimated to be 53 kDa on SDS-PAGE. The enzyme was successfully immobilized in calcium alginate gelatin composites in the presence of glutaraldehyde.
Structural Characterization and Enzymatic Activity of the Recombinant Ala959 To Ser1066 Region of Human Ace
Caroline Cristina Eliasa, Larissa Miranda Pereira, Danielle S. Aragão, Dulce E. Casarini, Regina Affonso*
Angiotensin-converting enzyme catalyzes the conversion of angiotensin I to the vasoconstrictor angiotensin II and the hydrolysis of bradykinin (BK). Human somatic angiotensin-converting enzyme has two homologous domains (N and C) that share 60% identity. Although these two regions have high homology, the catalytic site of the C-domain exhibits three-fold greater activity than the N-domain in the hydrolysis of angiotensin I in vivo.