Articles in Press 

December 2016

Volume 2  Issue 1

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Research Article

Molecular Cloning, Overexpression, Purification, Immobilization and Characterization
of Lipase from Staphylococcus pasteuri

Magdy M Yousef, H Elsawy^*

Thermostable lipase from Staphylococcus pasteuri was cloned, purified, immobilized and characterized. In this study, lipase gene was expressed in Escherichia coli and purified by DEAE-Sepharose and Glutathione S-sepharose 4B gel column chromatography. Lipase enzyme was purified to homogeneity by 28 times with a final yield of 175 %. The molecular weight of the purified enzyme was estimated to be 53 kDa on SDS-PAGE. The enzyme was successfully immobilized in calcium alginate gelatin composites in the presence of glutaraldehyde.