Шаблоны LeoTheme для Joomla.
GavickPro Joomla шаблоны

ENZYMEBANNER

Editorial

Recent Advances in Selection and Engineering of Biomass Degrading Enzymes


Alexander V. Gusakov *1, 2

1M.V.Lomonosov Moscow State University, Russia
2A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Russia

*Corresponding author: Dr. Alexander V. Gusakov ,Department of Chemistry, M. V. Lomonosov Moscow State University, Vorobyovy Gory 1/11, Moscow 119991, Russia; A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Pr. 33, Moscow 119071, Russia, Tel.: +7 495 939 5966; Fax: +7 495 939 0997;
E-mail: avgusakov@enzyme.chem.msu.ru

Submitted: 11-19-2014 Accepted: 11-19-2014  Published: 12-09-2014

Download PDF

_________________________________________________________________________________________________________________________

 

Article

 

Keywords: Biomass-Degrading Enzymes; Cellulase; Engineering; Lignocellulose; Selection

Plant biomass represents a renewable and practically inexhaustible bio resource for production of fuels and chemicals, and in the coming years it is considered to play the same role in human civilization as oil in 20th and beginning of the 21st century. For the last three decades, the production of first-generation liquid biofuels (mainly, ethanol from food crops in the USA and that from sugarcane in Brazil) has continuously grown [1]. Since 2012 an era of full-scale commercial facilities for production of second-generation biofuels, derived from lignocellulosic feedstock’s, began [1,2]. Enzymes, including cellulases, hemicellulases and auxiliary activities, are the most essential part of the second-generation biofuel technology [2,3]. Because of the recalcitrant nature of lignocellulosic biomass, demanding high enzyme loadings, much attention is paid in the laboratories worldwide to finding and selection of novel enzymes with higher activity, thermostability, less susceptibility to inhibition, etc., as well to engineering of already known enzymes in order to improve their properties.

Cellulases and hemicellulases produced by mutant strains of the filamentous fungus Trichoderma reesei (synonym Hypocrea jecorina) are the most studied and widely used biomass-degrading enzymes [2,3]. More active and thermostable cellulases than those secreted by Trichoderma have been discovered recently [2-9]. In particular, cellobiohydrolases from Myceliophthora thermophila (formerly name Chrysosporium lucknowense) [4], Acremonium thermophilum, Thermoascus aurantiacus, Chaetomium thermophilum [6,7] and Penicillium species (P. funiculosum, P. verruculosum, P. canescens) [5,8,9] display higher activity against microcrystalline cellulose (Avicel) and lignocellulosic substrates than cellobiohydrolase I of T. reesei (TrCel7A) at its optimal temperature (45-50oC). The last mentioned enzyme represents a major enzyme of T. reesei multienzyme cellulase system, a kind of a reference golden standard in the field of fungal cellulases [3]. Cellobiohydrolases from M. thermophila, T. aurantiacus, C. thermophilum are also characterized by higher thermostability than TrCel7A, and their superiority over TrCel7A becomes even more pronounced at temperatures above 50oC [4,6,7].

One of the most exciting recent events in the field of lignocellulose microbial degradation is a discovery of metal-dependent lytic polysaccharide monooxygenases that cleave cellulose via an oxidative mechanism [10,11]. These enzymes have recently been classified into family 9 of Auxiliary Activities (AA9) [12].The AA9 enzymes act as enhancers of cellulase activity, and now they become a standard component of commercial multienzyme cocktails for conversion of lignocellulosic biomass [13]. So, finding and selection of novel lytic monooxygenases that can effectively cleave cellulose and hemicelluloses, acting in synergism with true glycoside hydrolases, is now a very hot topic amongst researchers working in this area.

In parallel with finding more active cellulases and enzymes belonging to a new class of auxiliary activities, the good old enzymes of T. reesei have been subjected to engineering by site-directed mutagenesis or directed evolution in order to improve their activity/thermostability [14-16]. Using a combination of stabilizing mutations identified from homologous recombination, consensus design and structure- based computational approaches, the engineered T. reesei endoglucanase TrCel5A was obtained, having an optimal temperature higher by 17oC than the wild type enzyme [16]. Together with previously engineered thermo stable variants of cellobiohydrolases (TrCel6A and TrCel7A), TrCel5A was successfully applied for cellulose hydrolysis at 70oC over 60 h. The thermostable mixture produced 3-fold higher concentration of sugars than the best mixture of the wild type enzymes operating at its optimum temperature (60oC) [16].

An interesting approach for engineering of cellobiohydrolases with higher activity toward cellulose is adding or removing N-linked glycosylation motifs in the enzyme catalytic domain by site-directed mutagenesis [17]. Depending on the position of glycosylated Asn residue on the surface of enzyme molecule, the N-linked glycan may either reduce or stimulate cellulase activity. So, mutation of certain Asn residues to Ala in cellobiohydrolases  from T. reesei and P. funiculosum, thus removing N-glycosylation at the respective sites, resulted in up to 60% increase in conversion degree of bacterial crystalline cellulose after 120-h hydrolysis. Introducing a new glycosylation motif at N194 in P. funiculosum enzyme by applying A196S mutation also led to 70% increase in the enzyme activity [17].

Other engineering approaches have been focused on changing other properties of cellulases, such as pH-optimum of activity or susceptibility to inhibition. For example, using site-directed mutagenesis, the D98N mutant of endoglucanase III from P. verruculosum was obtained; as a result, the pH-optimum of enzyme activity was shifted from pH 4.0 to 5.1 [18]. As another case, chemical modification (succinylation) of T. reesei cellulases resulted in reduced inhibition of the enzymes by lignin; thus, more than 2-fold increase in the conversion of 1% Avicel in the presence of 1% lignin after 170-h hydrolysis was achieved [19]. The modified cellulases also displayed higher tolerance to ionic liquids.

The limits of this article do not permit more detailed analysis of the topic discussed. However, even a few examples presented above show that significant progress has been achieved in the last few years in the field of bioconversion of lignocellulosics through finding and selection of novel enzymes as well as engineering of enzymes, which had been discovered and studied previously.


Acknowledgements

This work was supported by the Russian Foundation for Basic Research (Grant No. 14-04-00724).

References

 References

1.Sims REH, Mabee W, Saddler JN, Taylor M. An overview of second generation biofuel technologies. Biores Technol. 2010, 101(6): 1570-1580.

2.Gusakov AV. Cellulases and hemicellulases in the 21st century race for cellulosic ethanol. Biofuels. 2013, 4(6): 567-569.

3.Gusakov AV. Alternatives to Trichoderma reesei in biofuel production. Trends Biotechnol. 2011, 29(9): 419-425.

4.Gusakov AV, Salanovich TN, Antonov AI, Ustinov BB, Okunev ON et al. Design of highly efficient cellulase mixtures for enzymatic hydrolysis of cellulose. Biotechnol Bioeng. 2007, 97(5): 1028-1038.

5.Gusakov AV, Sinitsyn AP. Cellulases from Penicillium species for producing fuels from biomass. Biofuels. 2012, 3(4): 463-477.

6.Viikari L, Alapuranen M, Puranen T, Vehmaanperä J, Siika-Aho M. Thermostable enzymes in lignocellulose hydrolysis. Adv Biochem Engin Biotechnol. 2007, 108: 121-145.

7.Voutilainen SP, Puranen T, Siika-aho M, et al. Cloning, expression, and characterization of novel thermostable family 7 cellobiohydrolases. Biotechnol Bioeng. 2008, 101(3): 515-528.

8.Morozova VV, Gusakov AV, Andrianov RM, Pravilnikov AG, Osipov DO et al. Cellulases of Penicillium verruculosum. Biotechnol J. 2010, 5(8): 871-880.

9.Volkov PV, Rozhkova AM, Gusakov AV, Sinitsyn AP. Homologous cloning, purification and characterization of highly active cellobiohydrolase I (Cel7A) from Penicillium canescens. Protein Expr Purif. 2014, 103:1-7.

10.Westereng B, Ishida T, Vaaje-Kolstad G, Wu M, Eijsink VG et al. The putative endoglucanase PcGH61D from Phanerochaete chrysosporium is a metal-dependent oxidative enzyme that cleaves cellulose. PLoS ONE. 2011, 6(11): e27807.

11.Quinlan RJ, Sweeney MD, Leggio LL, Otten H, Poulsen JC et al. Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components. Proc Natl Acad Sci USA. 2011, 108(37): 15079-15084.

12.Auxiliary Activity Family 9

13.Cannella D, Jørgensen H. Do new cellulolytic enzyme preparations affect the industrial strategies for high solids lignocellulosic ethanol production? Biotechnol Bioeng. 2014, 111(1): 59-68.

14.Nakazawa H, Okada K, Onodera T, Ogasawara W, Okada H et al. Directed evolution of endoglucanase III (Cel12A) from Trichoderma reesei. Appl Microbiol Biotechnol. 2009, 83(4): 649-657.

15.Lantz SE, Goedegebuur F, Hommes R, Kaper T, Kelemen BR et a. Hypocrea jecorina CEL6A protein engineering. Biotechnol Biofuels. 2010, 3:20.

16.Trudeau DL, Lee TM, Arnold FH. Engineered thermostable fungal cellulases exhibit efficient synergistic cellulose hydrolysis at elevated temperatures. Biotechnol Bioeng. 2014, 111(12): 2390-2397.

17.Adney WS, Jeoh T, Beckham GT, Chou Y-T, Baker JO et al. Probing the role of N-linked glycans in the stability and activity of fungal cellobiohydrolases by mutational analysis. Cellulose. 2009, 16(4): 699-709.

18.Tishkov VI, Gusakov AV, Cherkashina AS, Sinitsyn AP. Engineering the pH-optimum of activity of the GH12 family endoglucanase by site-directed mutagenesis. Biochimie. 2013, 95(9): 1704-1710.

19.Norwald EM, Brunecky R, Himmel ME, Beckham GT, Kaar JL et al. Charge engineering of cellulases improves ionic liquid tolerance and reduces lignin inhibition. Biotechnol Bioeng. 2014, 111(8): 1541-1549.

Cite this article: Gusakov A V. Recent Advances in Selection and Engineering of Biomass Degrading Enzymes. J J Enzymol Enzy Eng. 2014, 1(1): 001.

Contact Us:
9600 GREAT HILLS
TRAIL # 150 W
AUSTIN, TEXAS
78759 ( TRAVIS COUNTY)
E-mail : info@jacobspublishers.com
Phone : 512-400-0398